The COVID-19 pandemic underscored the urgent need for rapid and accessible diagnostics to detect emerging infectious diseases. Following a One Health approach, UniHealth addresses this challenge by developing easily adaptable, point-of-care solutions for human, animal and environmental samples. The Biosensors Lab at FORTH is leading this initiative and contributes to this mission by developing isothermal amplification assays for emerging viral threats, including influenza, SARS, Zika, Dengue, and West Nile virus. Our goal is to create highly sensitive, rapid, and reliable diagnostics that work directly with crude samples, eliminating the need for complex laboratory procedures. By optimizing universal reagents and employing bioinformatics-driven primer design, we aim to develop a versatile tool that can be easily adapted for the detection of any emerging threat, thereby enhancing global preparedness for future pandemics.